The Septuagint should not be confused with the seven or more other Greek versions of the Old Testament, most of which did not survive except as fragments (some parts of these being known from Origen’s Hexapla, a comparison of six translations in adjacent columns, now almost wholly lost). Of these, the most important are those by Aquila, Symmachus, and Theodotion.

The Septuagint derives its name from the Latin versio septuaginta interpretum, “translation of the seventy interpreters”, Greek: ἡ μετάφρασις τῶν ἑβδομήκοντα, hē metáphrasis tōn hebdomḗkonta, “translation of the seventy”. However, it was not until the time of Augustine of Hippo (354–430 CE) that the Greek translation of the Jewish scriptures came to be called by the Latin term Septuaginta.

The date of the 3rd century BCE is supported (for the Torah translation) by a number of factors, including the Greek being representative of early Koine, citations beginning as early as the 2nd century BCE, and early manuscripts datable to the 2nd century.

After the Torah, other books were translated over the next two to three centuries. It is not altogether clear which was translated when, or where; some may even have been translated twice, into different versions, and then revised. The quality and style of the different translators also varied considerably from book to book, from the literal to paraphrasing to interpretative.

The translation process of the Septuagint itself and from the Septuagint into other versions can be broken down into several distinct stages, during which the social milieu of the translators shifted from Hellenistic Judaism to Early Christianity. The translation of the Septuagint itself began in the 3rd century BCE and was completed by 132 BCE, initially in Alexandria, but in time elsewhere as well.

The Septuagint is the basis for the Old Latin, Slavonic, Syriac, Old Armenian, Old Georgian and Coptic versions of the Christian Old Testament.